Andreu Ferrer - Martínez and Anna
نویسنده
چکیده
Gene targeting or trapping constructs that utilize the lacZ gene encoding β-galactosidase activity to trap promoter expression have become an increasingly important way to disrupt gene function and monitor gene expression. A number of genes targeted in this way have revealed both expected and unexpected developmental abnormalities of the skeleton. The use of X-gal staining to monitor gene expression in developing skeletal structures is hampered in these mutants because, during the critical latter stages of mouse embryonic development, visualization is hindered by the opacity of overlying soft tissue. Here, we report the development of a reliable method to clear exogenous tissue in late-stage embryos and neonates that still preserves skeletal X-gal staining patterns. This protocol reveals (i) specific cell staining in localized regions of developing bone and cartilage in two different genetic models and (ii) that the intensity of X-gal staining is consistent with the level of expression of lacZ. We conclude that this protocol accurately reflects both the specificity and intensity of expression and will facilitate the analysis of mouse skeletal development.
منابع مشابه
The UPV-PRHLT Combination System for WMT 2010
Jesús González-Rubio and Jesús Andrés-Ferrer and Germán Sanchis-Trilles Guillem Gascó and Pascual Martı́nez-Gómez and Martha-Alicia Rocha Joan-Andreu Sánchez and Francisco Casacuberta Instituto Tecnológico de Informática Departamento de Sistemas Informáticos y Computación Universidad Politécnica de Valencia {jegonzalez|jandres|gsanchis}@dsic.upv.es {ggasco|pmartinez|mrocha}@dsic.upv.es {jandreu|...
متن کاملUPV-PRHLT English-Spanish System for WMT10
Germán Sanchis-Trilles and Jesús Andrés-Ferrer and Guillem Gascó Jesús González-Rubio and Pascual Martı́nez-Gómez and Martha-Alicia Rocha Joan-Andreu Sánchez and Francisco Casacuberta Instituto Tecnológico de Informática Departamento de Sistemas Informáticos y Computación Universidad Politécnica de Valencia {gsanchis|jandres|fcn}@dsic.upv.es {ggasco|jegonzalez|pmartinez}@dsic.upv.es {mrocha|jand...
متن کاملLaser scanning cytometry to quantify gene transfer efficiency and transcriptional activity of EGFP constructs.
Enhanced green fluorescent protein (EGFP) is the preferred reporter protein for real-time detection in individual cells, but its usefulness for gene expression quantification is limited by the sensitivity of standard detection techniques. We tested whether the unique feature of single-cell detection and quantification by laser scanning cytometry permits the evaluation of EGFP gene expression in...
متن کاملAutonomous navigation for mobile service robots in urban pedestrian environments
E. Trulls, A. Corominas Murtra, J. Pérez-Ibarz, and G. Ferrer Institut de Robòtica i Informàtica Industrial, CSIC-UPC, Barcelona, Spain e-mail: [email protected], [email protected], [email protected], [email protected] D. Vasquez Swiss Federal Institute of Technology, Zürich, Switzerland e-mail: [email protected] Josep M. Mirats-Tur Cetaqua, Centro Tecnológico del Agua, Barcelona, S...
متن کامل